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Back to Eccles Lab > DGG Protocols

cDNA Synthesis:

Part 1

  1. 65°C for 5 minutes (Called RT1 in Shujie's folder on red PCR machine).
  2. Put on ice.

Part 2

  1. Add to the above RNA solution.
  2. 25°C for 5 minutes.
  3. 50°C for 60 minutes.
  4. 70°C for 15 minutes (Called RT2 in Shujie's folder on red PCR machine).