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Contents

Protein Lysates from Tissue

Cell Lysis Buffer

5mL 0.1M Tris HCl pH 8 (10mM)
0.44g NaCl (150mM)
0.02g EDTA (1mM)
0.5mL nonidet P40 (1% w/v)
0.05g SDS (0.1% w/v)
Make up to 50mL with MQH2O and filter sterilise.

20x Complete mini Protease Inhibitor

Take 1 tablet and dissolve in 0.5mL MQH2O by pipetting up and down.
Store on ice.

Dilute 20 fold in cell lysis buffer

Cell Lysis Buffer containing 1x Complete Protease Inhibitor

50uL 20x Complete protease inhibitor
950uL Cell Lysis Buffer


If your tissue is relatively soft and small (eg; mouse kidney, liver, brain etc) then the tissue can be crushed in Cell Lysis Buffer using an eppendorf and pestle on ice.
If you are dealing with larger or fibrotic tissue samples then these are best crushed using a mortar and pestle under liquid nitrogen.

Eppendorf and Pestle Tissue Disruption

Mortar and Pestle Tissue Disruption using Liquid Nitrogen