NOTICE: You are viewing a page of the openwetware wiki. Our "dewikify" feature makes a wiki page appear as a normal web page. In April 2017, this feature will GO AWAY and this URL will redirect to the source URL on our wiki. We're sorry for the inconvenience.

Collagen Coating of Cell Culture Vessels

Vessel Size Flask/Well Surface Area (cm2) Media Volume Volume Collagen Solution (0.05ug/ul) to Achieve ~6ug/cm2
96 well plate 0.280.2ml33ul
24 well plate 2.00.5ml300ul
6 well plate or 35x10mm round dishes 9.62ml1.1ml
T25 flask 255ml2.9ml
T75 flask 7520ml8.8ml
T175 flask 17550ml20.6ml

Collagen Solution (0.05ug/ul)

Collagen Stock (1ug/ul) 1x PBS
Volume (to make 50ml) 2.55ml47.45ml