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Collagen Coating of Cell Culture Vessels


Vessel Size Flask/Well Surface Area (cm2) Media Volume Volume Collagen Solution (0.05ug/ul) to Achieve ~6ug/cm2
96 well plate 0.280.2ml33ul
24 well plate 2.00.5ml300ul
6 well plate or 35x10mm round dishes 9.62ml1.1ml
T25 flask 255ml2.9ml
T75 flask 7520ml8.8ml
T175 flask 17550ml20.6ml

Collagen Solution (0.05ug/ul)


Collagen Stock (1ug/ul) 1x PBS
Volume (to make 50ml) 2.55ml47.45ml